A Present for Charlie

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Diabetes 50(8): 1691–1697. J. B. (2006). Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 367(9518): 1241–1246. , Massumi, M. K. (2006). Generation of insulin-secreting cells from human embryonic stem cells. Dev. Growth Differ. 48(5): 323–332. , Santa-Olalla, J. and Covarrubias, L. (2003). Neural stem cells in development and regenerative medicine. Arch. Med. Res. 34(6): 572–588. P. M. (2004). Mesenchymal stem cells: clinical applications and biological characterization.

Cells not yet fully differentiated and capable of significant proliferation) can be obtained from a small biopsy of a patient’s tissue, and these cells are able to expand and differentiate in culture and/or after implantation back into the patient, autologous therapies are feasible. These have the great advantage of avoiding the risk of immune rejection based on differences in histocompatibility antigens, so that the use of immunosuppressive drugs is not required. However, there is a substantial practical appeal to “off the shelf ” products that do not require the cost and time associated with customized manufacturer of an individual product for each individual recipient (Lysaght and Hazlehurst, 2004).

Bioreactor cultivation enhances the efficiency of human embryoid body (hEB) formation and differentiation. Biotechnol. Bioeng. 86(5): 493–502. 11 12 INTRODUCTION TO REGENERATIVE MEDICINE Gimble, J. and Guilak, F. (2003). Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5(5): 362–369. L. and Song, H. (2003). Adult neural stem cells and repair of the adult central nervous system. J. Hematother. Stem Cell Res. 12(6): 671–679. , Young, L. and Denning, C.

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