Advances in pectin and pectinase research by J.-C. Mollet, S.-Y. Park, E. M. Lord (auth.), Fons Voragen,

By J.-C. Mollet, S.-Y. Park, E. M. Lord (auth.), Fons Voragen, Henk Schols, Richard Visser (eds.)

The second one foreign symposium on Pectins and Pectinases was once organised by way of Wageningen college and learn Centre and used to be held in Rotterdam, could 6-10, 2001. This fruitful assembly was once attended through round a hundred thirty members from greater than 20 nations, representing just about all of the groups/industries operating around the globe on pectins and pectinases. Following the 1st assembly in this topic held in December 1995, the symposium certainly kinds a platform for researchers and industries operating within the box, all inside their very own self-discipline and services. The symposium publication comprises so much keynote lectures and different oral shows and gives an replace concerning the present study. it really is truly confirmed that major growth has been made in past times seven years. The development within the elucidation of the chemical constitution of pectin and mode of motion and three-D constitution of the pectin-degrading enzymes permits us a growing number of to spot (and impact) the performance of pectins and pectic enzymes, either in vitro after isolation in addition to within the vegetation themselves (in planta). different contributions take care of new functions of either pectin and pectin-degrading enzymes, whereas progressively more realization is paid to well-being and dietary points of pectins.

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C. (2000) A family of at least seven -galaetosidase genes is expressed during tomato fruit development. , 123 , 1173-1183. , Bacie, A. E. (1998) Hydroxyproline-rieh plant glyeoproteins. Phytochemistry 47, 483-497. , Bush, M. , MeCann, M. , and Ulvskov, P. (2000) 34 Pectin engineering: Modification of potato pectin by in vivo expression of an endo-1,4- -D-galactanase. Proc. Natl. Acad. Sei. USA, 97, 7639-7644. Thakur, B. , Singh, R. , and Handa, A. K. (1997) Chemistry and uses ofpectin. Crit. Rev.

2000) 34 Pectin engineering: Modification of potato pectin by in vivo expression of an endo-1,4- -D-galactanase. Proc. Natl. Acad. Sei. USA, 97, 7639-7644. Thakur, B. , Singh, R. , and Handa, A. K. (1997) Chemistry and uses ofpectin. Crit. Rev. , 37, 47-73. 8. (1999) Molecular and genetic characterization of a novel pleiotropic tomato-ripening mutant. Plant Physiol. 120,383-389. , Knox, J. , and Driouich, A. (1998) Immunolocalization of 1 ~)- and 1~6)-D galactan epitopes in teh cell wall and Golgi stacks of developing flax root tissues.

Due to the inhibition of nonnal stolon and tuber development in the arabinanaseexpressing potato plants, we have not been able to show, by using conventional biochemical techniques and immunogold labeling with the arabinan specific antibody LM6, that apoplastic expression ofthe endo-arabinanase results in a changed RGI sugar composition or epitope abundance. The carbohydrate moieties of cell wall associated proteoglycans mayaiso be hypothetical substrates for the arabinanase when expressed to the apoplastic space.

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